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To ensure that this was indeed the case, WT, REW and PGLALA anti-NIP IgG1 variants were coated directly in ELISA wells in parallel with an IgG1 variant containing the E345R/E430G/S440Y (RGY) amino acid substitutions, which is known to form solution phase hexamers 40

In the latter situation, it could be that GH-mediated enhancement of aromatase activity leads to an increase in testosterone to E2 conversion, reducing testosterone levels but increasing E2 (72, 88)

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