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Suspensions were centrifuged at 5,000 g for 5 min at 4C, supernatants were transferred to a new tube, and TCA buffer was added to a final concentration of 33% and incubated at 4C overnight to precipitate histone proteins

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casei Zhang significantly reduced serum cystatin C and parathyroid hormone levels in non-dialytic CKD patients (stages 3 to 5) after 3 months of administration

10.1128/IAI.69.6.3685-3691.2001 228

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