Intracellular metabolites (GSH, glucose, glucose-6P, pyruvate, succinate) were measured by liquid chromatography (Vanquish HPLC Thermo Scientific) coupled to high resolution mass spectrometry (QExactive HF Thermo Scientific)
The two protocols do not overlap and can be run in adjacent cycles under provider guidance
In addition, adults also have positive correlations with functions in protein fate, central intermediary metabolism, and biosynthesis of cofactors, prosthetic groups and carriers, as well as a negative correlation with the pentose phosphate pathway within energy metabolism
Because those signals converge on appetite from different directions, the hypothesis is greater total reduction in energy intake than either alone
Effects of glucose-dependent insulinotropic peptide on behavior