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The wells were blocked with DPBS (pH 7.4)/4% (w/v) skimmed milk powder (Sigma-Aldrich) (PBS/M) (250 L/well) for 1 h at room temperature and washed three times with PBS/0.05% (v/v) Tween 20 (PBS/T) at either pH 5.5 or pH 7.4

Endogenous cardiotonic steroids: physiology, pharmacology, and novel therapeutic targets

GLP- 1's anti-apoptotic and proliferative effects are also mediated by the PI3 K/Akt axis through the transactivation of the epidermal growth factor receptor (EGFR), which is connected to GLP- 1 receptor through c-Src activation and the generation of endogenous EGF like ligands [59]

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Take our Peptide Quiz: Get personalized recommendations in 2 minutes BPC-157 Dosage Calculator: Find your optimal protocol How to Inject Peptides: Step-by-step technique guide BPC-157 Complete Guide: Everything about this peptide References 1.Sikiric P, et al