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In theory, direct PKA-driven activation of uncoupling proteins may accelerate mitochondrial fat oxidation

We separated nuclear and cytoplasmic proteins and calculated the ratio of p-P53 to total P53 in each fraction

The insoluble portion was subsequently removed by centrifugation and the supernatant was incubated with Ni-NTA resin (GE Healthcare) for 2 h batch binding at 4 C

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30 mg vial reconstitution chart For the 30 mg vial, the 10 mg/mL concentration (3.0 mL water) is the gold standard