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Mechanisms of gut microbiota modulation: Investigating the influence of the gut microbiota on the defense system against ferroptosis and the signaling pathways through which the gut microbiota regulates ferroptosis

Measurement of reactive oxygen species (ROS) and reduced glutathione (GSH) After maturation, the oocytes were incubated in 10 M CM-H 2 DCFDA (Thermo Fisher Scientific, Barcelona, Spain) and 10 M Cell Tracker Blue (Thermo Fisher Scientific, Barcelona, Spain) for 30 min at 37 C in the dark to detect intracellular reactive oxygen species (ROS) and reduced glutathione (GSH), respectively

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Yuen GS, Bhutani S, Lucas BJ, Gunning FM, Abdelmalak B, Seirup JK, et al