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( D ) Intracellular ROS levels were measured by CellROX and DCFDA staining while mitochondrial ROS levels by MitoSOX in TNBC 4T1 series cells ( n = 3 biological replicates in either single or technical duplicate)

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The data was then normalized to DAPI area to account for differing numbers of cells within respective imaging areas

This activity of GLP-1 has been demonstrated in both preclinical and clinical studies, and the results support the hypothesis that the GLP-1 pathway regulates the processes of alcohol use disorder (AUD)