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This inflammatory process includes microglial activation, increased cytokine release, and increased oxidative stress, as well as changes in glutamatergic system regulation that may exacerbate astrocyte atrophy and local damage following these events (48)

After OGD treatment, the number of Ki-67-positive endothelial cells significantly decreased, while the intracellular expression levels of senescence markers P21, P53, and the DNA damage indicator -H2AX were markedly elevated (Figure 3B)

Glutathione is a tripeptide commonly used in tissues to detoxify free radicals and reduce cellular oxidation