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Liver fractions were thawed on ice and diluted in 0.05 M potassium phosphate buffer (pH 7.4) to a final protein concentration of 1.25 mg/mL Test compounds ( 11 - 15 ) were dissolved in DMSO to create stock solutions of 5 mM

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Their conclusion: muscle-based losses exceeded those benchmarks in 68% of incretin-based interventions