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For protocols in the 1-2.5 mg range, 3 mL keeps injection volumes manageable at 10-25 units per dose

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As far as peptide 20 is concerned, the peptide binding pocket of both GLP-1R and GCGR closely resembled that of GLP-1 and GCG bound structures, where notable conformational change was only observed in the ECL1 of GIPR