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Hence, a peptide was designed by substituting the negatively charged residues D42, D48, D49, E51, E56, and D57 within the p53 TAD PEP with alanine to increase its binding affinity to FOXO4 FHD and enhance membrane penetration

This helps ensure the efficacy and quality of the product being taken and minimizes the risk of positive drug testing for steroids and other banned substances

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10.1016/j.jchromb2016.02.015 J Chromatogr B Analyt Technol Biomed Life Sci

WADA prohibits the administration of these substances when used to artificially raise levels beyond normal physiological ranges for the purpose of performance enhancement.