Severe deficiency : Weekly or biweekly injections may initially be required to correct anemia and neurological symptoms
A subcutaneous injection is an injection thats given under your skin
It is non-toxic and relatively safe, with possible side effects including nausea, indigestion, orthostatic hypotension, and blurred vision
For Safranin O/Fast Green staining, slides were stained with hematoxylin (Mayer, Sigma) for 5 min, 0.005% Fast Green (Thermo Fisher Scientific, Pittsburgh, PA, United States) for 1 min, rinsed with 1% acetic acid, and stained with 0.1% Safranin O (Millipore, Billerica, MA, United States) for 20 min
Below are key recommendations for incorporating the PSPen into a structured laboratory protocol