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Automated data acquisition was performed using SerialEM 50 on a K3 Summit direct detection camera (Gatan) in superresolution correlated double-sampling mode with a pixel size of 0.52 and a defocus range of 0.9 to 2.2 m

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Several membrane-bound proteins suggested to play a role in this process include CD73, able to convert NMN into NR 67 , and NAD + glycohydrolases such as CD38 that convert NAD + into NAM and ADP-ribose