Nitrotetrazolium blue chloride (NBT) N360 or oncology models

or oncology models

Key Features and Advantages

Formula: C8H11NO5S

DNA damage

How It Works: The hot-start polymerase and proprietary buffer chemistry reduce primer-dimer formation and nonspecific amplification

Nitrotetrazolium blue chloride (NBT) N360 or oncology modelsNitrotetrazolium Blue (NBT) is used in combination with 5 bromo 4 chloro 3 indoxyl phosphate (BCIP) to detect alkaline phosphatase activity in blotting techniques and in situ hybridization chemistry. BCIP acts as the substrate for alkaline phosphatase, and the oxidizing reagent NBT enhances the purple brown final color of the precipitates at the reaction sites. NBT has also been used with X Gal,or Salmon Gal to speed up and enhance colony screening.