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After incubation for 24 h to reach 80% confluence, the cells were transiently transfected with GLP-1R-Rluc8 and -arrestin 1/2-Venus at a 1:3 mass ratio using lipofectamine LTX reagent (Invitrogen) and cultured for 24 h

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Glutaminase and poly(ADP-ribose) polymerase inhibitors suppress pyrimidine synthesis and VHL-deficient renal cancers