4-Nitrophenyl-N-acetyl-β- D-glucosaminide P303 Add 10 mL sterile H2O

Add 10 mL sterile H2O

·         Protocol Compatibility: Compatible with standard LB agar

GoldBio may require shipment by Next Day Air with blue ice in order to avoid high temperatures that may adversely affect DTT quality

Purpose: To isolate GST-tagged bait proteins together with interacting binding partners

You should usually add IPTG at the mid-log phase (OD600 ≈ 0

4-Nitrophenyl-N-acetyl-β- D-glucosaminide P303 Add 10 mL sterile H2O4 Nitrophenyl N acetyl D glucosaminide is a chromogenic Glucosaminidase substrate, yielding a yellow solution upon cleavage, used particularly in yeasts and molds. Product Specifications 4 Nitrophenyl N acetyl D glucosaminide p Nitrophenyl 2 acetamido 2 deoxy D glucopyranoside PNP D GlcNAc MOLECULAR BIOLOGY GRADE Formula: C14H18N2O8 MW: 342. 30 g mol Storage Handling: Store at 20C. Protect from light. Pubchem CID: 102416 Literature: 1. C. S. Jones, D.