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Recent trial of proplylactic treatment with T 4 (1.0 ~ 2.0g/Kg/day) for one year in euthyroid patients with Hashimoto's thyroiditis showed decrease of anti-TPO antibodies and thyroid B-lymphocytes(105), suggesting prophylactic T 4 therapy might be useful to stop progression of disease

After washing once with PBS to remove unbound CFSE, SFN was added for 30 min and the cells were seeded on 96-well microplates coated with anti-CD3/CD28 antibodies for co-stimulation as described above

Bafilomycin A1 disrupts autophagic flux by inhibiting both V-ATPase-dependent acidification and Ca-P60A/SERCA-dependent autophagosome-lysosome fusion

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