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MOR is widely expressed in various brain regions, including the nucleus accumbens, ventral tegmental area, amygdala, cerebral cortex, and hippocampus 23,24

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The next day, the membranes were washed three times with TBST and then incubated with anti-rabbit IgG conjugated with IRDye 800CW (LI-COR Biosciences, Lincoln, Nebraska, USA) for 1 h at RT