The supernatant (300 L) was cryopreserved at 20 C for 30 min and recentrifuged (12,000 rpm, 4 C, 3 min), and 200 L aliquots were analyzed with LC-MS/MS (Waters Xevo TQ-S)
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They write that it has been linked with serious cardiovascular conditions such as dyslipidemia, myocardial infarction, arrhythmia, atherosclerosis, cardiomyopathy and left ventricular hypertrophy as well as significantly higher coronary artery plaque volume and reduced ejection fraction compared to non-using controls. With testosterone, there are a bunch of adverse side effects, said Chandler
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Statistical analysis All results were expressed as mean standard deviation (SD), with experiments performed using at least three biological replicates
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