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These findings were further validated in OCY454 osteocyte-like cells, a well-established in vitro model of osteocytes [20]

Notably, in these studies, the upregulation of GPx4 has been achieved by using exogenous agents (like NVP-AUY922, Bafilomycin A1, PD151746, GsMTx4, epigallocatechin-3-gallate, total flavonoids of Engelhardia roxburghiana leaves, Lycium barbarum polysaccharide-glycoprotein, perillaldehyde, melotonin, ferulic acid, and sphingosine-1-phosphate) which either inhibit the degradation of GPx4 or increase its transcription by activating the Nrf2 [67]

The downstream chain (transport, mitochondrial preference, NAD+ cofactor capacity) determines whether mobilized fat becomes usable energy

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