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Protein extraction and immunoblot analysis Total protein from 16-HBE cells was extracted using ice-cold radioimmunoprecipitation assay buffer supplemented with 1 mM phenylmethanesulfonyl fluoride (PMSF) (Thermo Fisher Scientific) or phosphatase inhibitor cocktail (Calbiochem, La Jolla, CA, USA) for phosphoprotein

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References: JAMA Internal Medicine .

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The physical aggression of the neighbour and the cultural invasion of the west (in the form of Chopra) were viewed equally inimical to our swadeshi ideals