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De-paraffinization and tissue rehydration for all staining procedures was achieved by immersing the glass slides through the following solutions: three 15 min xylene washes, two 5 min washes of 100% ethanol and 95% ethanol, and a 5 min wash of 70% ethanol, 50% ethanol, and dH 2 O

It refers to a condition where people may become dependent or even addicted to alcohol, tobacco or other types of drugs (either street drugs or prescription medications)

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