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Immunohistochemistry Cells were fixed in 4% paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with PBS supplemented with 1% bovine serum albumin

According to Mayo Clinic, quitting tobacco can: slow your heart rate just 20 minutes after your last cigarette reduce the levels of carbon monoxide in your blood to a normal range within 12 hours improve your circulation and lung function within 3 months cut your risk of a heart attack by 50 percent within a year reduce your stroke risk to that of a nonsmoker within 5 to 15 years Quitting smoking can be tough, but its possible

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