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The membrane was washed three times in Tris-buffered saline with Tween 20 (TBS-T) for 10 min, and treated with HRP-conjugated anti-rabbit or anti-mouse IgG (GE Healthcare) for 1 h

Other strategies would involve using inhibitors that target Nrf2 such as, the quassinoid brusatol 79 , the alkaloid trigonelline 80 and the flavonoid luteolin 81 , all of which showed efficacy in inhibiting Nrf2 activity and prevented chemo-resistance

The acetaldehyde produced by the ADH reaction, as well as the ROS produced via CYP2E1 oxidation, both can interact with proteins and other biomolecules in the cell to form both stable and unstable adducts