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Furthermore, we carried out unsupervised clustering by constructing a shared nearest neighbor (SNN) graph based on k -nearest neighbors ( k = 20) and then determining the number and composition of clusters using a modularity function optimizer based on the Louvain algorithm (resolution = 0.22)

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Graphs were plotted using GraphPad Software (GraphPad, San Diego, CA.), and significance was obtained using a one-way analysis of variance (ANOVA) followed by Dunnetts post hoc test in which treated groups were compared to the lowest concentration