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Afterward, the membranes underwent 3 10 min washes with TBST and were subsequently exposed to secondary antibodies diluted in 0.05% TBST (refer to Supplementary Table S1) for one hour at room temperature

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By this equation, smoking was the longest lived addiction, with the largest percentage (though still only a small minority) continuing into old age

Improve ventilation: Open windows and doors to air out the space