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To eliminate technical variability during acquisition, individual samples within one experiment were fixed with Maxpar Fix I Buffer for 10 min at RT, permeabilized with Maxpar Barcode Perm Buffer, and barcoded with the Cell-ID palladium (Pd) barcoding kit (Fluidigm) for 45 min at RT

The HepG2 cell line was gifted by Xianghua Yans lab (Huazhong Agricultural University)

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