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Human primary blood-derived monocytes (pBDMCs) were isolated from donor buffy coats using the EasySep Direct Human Monocyte Isolation Kit (Stemcell, #19669), which isolates primary CD14 + monocytes via negative immunomagnetic selection according to the manufacturers protocol (Supplementary Fig

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In cases 1 and 14, despite lower eosinophil counts, their clinical symptoms and radiological evidence were consistent with AEP, justifying their inclusion in the study

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Smoking Initiation The onset and progression of tobacco use among young people, from adolescence into young adulthood, is a dynamic, multistage process influenced by multiple determinants