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Thereafter, sulfosalicylic acid solution (35 g/L, 15 mL) was added, vortexed and centrifuged (10500 r/min, 20 min)

NRVMs were exposed to different concentrations of nicotine for 48 h, and cleaved caspase-3 and cleaved PARP were significantly increased with nicotine treatment (Figures 6AC)

The combination of nicotine replacement therapy (NRT) and behavioral support is the recommended best practice for quitting by the medical community at large

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