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To determine toxic doses of E-liquid, A549 cells were treated with 2 mL of the following concentrations of E-liquid minus nicotine: 0, 0.22, 2.2, 11, 22 & 44 mM (0, 0.1, 1, 5, 10 & 20%, respectively)

However, the researchers say the toxins likely have an impact on other forms of aquatic life too, with previous studies showing adverse effects on fish , even at relatively small concentrations

Quantitative analysis revealed that the increase in the relative expression level of TRPM8 measured as positive cells by immunostaining for the M-CS-, Non-M-CS-, and air-exposed groups were 5 0.1 ( n = 9), 2.2 0.1 ( n = 9), and 1.0 0.1 ( n = 9) folds, respectively