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However, smoking lost its popularity later in the 20th century, given that people became more aware of its unhealthy effects on the body

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To build the alveolar models, NCI-H441 cells were cultured on pre-coated semi-porous (0.4 m diameter) transwell inserts (BD Falcon, USA) in 12-well plates with a seeding density of 250,000 cells/cm 2

Health officials have been concerned that teens and other young adults may view e-cigarettes as an alternative to cigarettes with a rising number of teens using e-cigarettes

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