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References: Castelli, G., Desai, K

As we age, glutathione levels can decrease due to stress, environmental toxins, and lifestyle factors

To assess the number of redox-sensitive Cys residues in GSAAT, the enzyme was treated with hydrogen peroxide or DTT (0.110 mM DTT) and subsequently with N-ethylmaleimide (NEM) to irreversibly block the free thiol groups of Cys residues in GSAAT

Cell Mol Life Sci:120 Ghanizadeh A, Moghimi-Sarani E (2013) A randomized double blind placebo controlled clinical trial of N-acetylcysteine added to risperidone for treating autistic disorders

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