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The slide was immediately dipped in cold (20C) methanol in a Coplin jar for 1 min, and then transferred to 1X PBS buffer (10 mM Phosphate pH 7.4, 137 mM NaCl, 2.7 mM KCl) containing 0.1% Tween-20 (PBST) at room temperature

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Milestone moment PML says it reached a major milestone in 2023 as global sales of IQOS overtook those of its Marlboro brand for the first time