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Preliminary experiments The purposes of the experiments were to determine the optimum pH of the buffer solution for non enzymatic and enzymatic reactions of monobromobimane (MBB) with glutathione (GSH) as well as the quenching effect of the presence of excess MBB in a typical reaction mixture on the fluorescence of the reaction product

The substrate-binding site is located in the solvent-exposed cleft between the thumb subdomain and the palm subdomain, which possess a catalytic triad composed of C111, H272 and D286

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10.1016/j.drudis.2016.01.013 5 AugestadI