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What the researchers uncovered was a patchwork of practices and outcomes that raised red flags

doi: 10.1136/rmdopen-2014-000012

Tissue homogenization: Frozen tissue of brain was homogenized in 9 volumes of ice-cold tissue lysis buffer containing 150 mM sodium chloride, 1.0% NP-40, 50 mM Tris, pH 8.0 and 1 mM PMSF (phenylmethane sulfonylfluoride) with protease inhibitors (Sigma, Singapore)

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So thats kind of where we went. Theyd evaluated their stores, which included two that were profitable and others close to breaking even, but they were scattered throughout the U.S., so they opted to take over the location in Sioux Falls from corporate at 1900 S