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Handling should be performed only by qualified personnel.

DH supervised the study and revised the manuscript

Hepatic concentrations of active sulfate, in the form of PAPS (adenosine-3'-phosphate 5'-phosphosulfate) were also decreased and could be restored to normal by supplementation with methionine [13]

Moving forward, refining in vivo techniques to enhance reproducibility and reduce ethical concerns will be vital for ensuring reliable translation of research findings into clinical and therapeutic applications (Zahra et al., 2022