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Using human fetal lung WI-38 fibroblasts induced to senesce via replicative exhaustion, we report a novel role for acid ceramidase (ACase), which breaks down ceramides into sphingosine and free fatty acids, in regulating the sensitivity of senescent cells to RSL3-induced lipid peroxidation and ferroptosis through the modulation of polyunsaturated fatty acid composition of membrane phospholipids

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It promotes the conversion of liver glycogen to glucose and increases gluconeogenesis, as well as enhancing fat breakdown to provide energy

These data suggest that the anticoagulant used for sample collection and time to deproteinization, both independently and additively impact measured levels of free GSH (and thus the ratio of free GSH to GSSG) in samples of plasma which are deproteinized and stored at 80 o C