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(The trial was funded by Novo Nordisk, which sells semaglutide as Ozempic for diabetes and Wegovy for obesity.) Remarkable

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doi: 10.12691/jfnr-3-9-6 6 StefaniMRigacciS

Post-blocking blots were incubated overnight at 4 C on a tube roller (BioCote Stuart SRT6) with primary antibodies against total ubiquitin P4D1 (1:1000, Santa Cruz #sc8017), Lys-48 specific anti-ubiquitin antibody (1:1000, Sigma Aldrich #05-1307), NRF2 (1:2000, Proteintech #16396-1-Ap), Tubulin (1:5000, Proteintech #66031-1-Ig) or Streptavidin-HRP (1:20,000, Abcam #ab7403) in an enzyme dilution buffer containing 0.2% BSA (w/v) and 0.1% Tween20 (v/v) in PBS

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