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Subsequently, 0.4 mL of a PI staining/lysis solution (0.5% NP-40, 0.5 mM ethylenediaminetetraacetic acid [EDTA]) and 50 L of RNase A (10 mg/mL in TrisEDTA buffer, pH 8.0) solution were added

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AOD-9604 was developed to: Stimulate lipolysis (breakdown of stored fat) Inhibit lipogenesis (formation of new fat) Avoid raising IGF-1 levels Reduce impact on blood glucose Because it is a small fragment rather than the full hormone, it theoretically targets fat metabolism more specifically

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