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Insect whole body specimens and faeces (1 g representative sample was made from 5 larvae) were homogenized and extracted in a buffered acetonitrilewater (0.5 mL:0.5 mL) extraction and this extract is added to the Agilent AOAC extraction kit and is vortexed for 5 s (pH was adjusted to 11 by using NaOH) and centrifuged or 5 min at 5000 rpm

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Thus, 285 participants allocated to the CASC group and 287 participants assigned to the control group were included in the full analysis set