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A TUE is only granted when the following four key criteria are met: It is a necessary treatment for a diagnosed medical condition Its unlikely that the treatment will improve your performance There is no reasonable permitted alternative The requirement to use the substance or method is not due to the prior use of substance or method without a valid TUE

Materials and methods 2.1 Bacterial strains and growth conditions The mutagenesis procedure [17], DNA transformation procedures [18], growth conditions [19], and stock preparation of the pneumococcal strains [20] have been previously reported