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But it also marked the end of an extraordinary relationship between the reserved and refined former first lady and the free-spirited singing sensation 16 years her junior

Ultrathin sections were then prepared using an ultrathin microtome (Leica EM UC6, Wetzlar, Germany), double-stained with uranyl acetate and lead citrate, and observed and photographed using a transmission electron microscope (JEM-1400 Plus, Japan), with ten fields of view for each treatment (Xin et al., 2024)

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That insistence made Charles one of the most provocative painters to emerge in the 1990s

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