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Green regions on predicted truncated proteins denote ectopic amino acid sequences not found in wild-type TCF7L2 protein

Finally, ARG2 concentration was determined at 450 nm wavelength by EMax Plus microplate reader (Molecular Devices, CA)

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Approximately 1.3 million deaths per year are attributed to second-hand smoke exposure and in the UK around 10,000 deaths a year (statistics provided by The World Health Organisation WHO)

However, their integration into harm reduction strategies must be accompanied by continuous surveillance, transparent reporting, and rigorous long-term research to evaluate safety, usage patterns, and population-level health outcomes