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The BD PE Annexin V Apoptosis Detection Kit was used for apoptosis determination (BD Pharmigen, #579563)

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Glutathione is a naturally occurring tripeptide made up of three amino acids: cysteine, glutamine, and glycine

Since MTX, one of the DMARDs used in clinical practice, showed no obvious influences on the differentiation of osteoclasts (Supplementary Fig

You need: one vial of lyophilized glutathione, one vial of bacteriostatic water (USP grade, 0.9% benzyl alcohol), alcohol prep pads, one 3mL or 5mL sterile syringe, and one 18-gauge drawing needle