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It's status as an artifact also comes with the usual pluses and vulnerabilities

The change in intracellular calcium ion concentration was represented by F, calculated using the following formula: where F 0 represents the fluorescence intensity in the CLSM image of the FITC channel before the sample addition, and F t represents the maximum fluorescence intensity in the CLSM image of the FITC channel obtained within 3 min after the sample addition

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