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Maximum Dose of 30 ml For Body Using 30G needle, 1.5 to 2ml with point interval of 1.5 to 2 cm

The column and the detection cell were housed within the Faraday cage of the electrochemical detector (25.5 C)

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Ethics statement The studies involving humans were approved by the University of Surrey Ethics Committee and a favorable ethical opinion was granted, reference FHMS 2122,026 EGAAmend 3