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For Flag-PARP1 purification, HEK293T cells were transfected with Flag-PARP1 for 72 h, then were lysed in lysis buffer (25 mM Tris pH 7.5, 150 mM NaCl, 1% [v/v] NP-40, 5 mM EDTA, 5% [v/v] glycerol, 10 mM -glycerophosphate, 5 mM NaF, 1 mM DTT, 0.1 mM Na 3 VO 4 , and protease inhibitor cocktail)

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