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In this study, which was published in Tobacco Control, 28 HWLs were developed

JSTOR 10.3098/ah.2015.089.2.200

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Determination of physiological, biochemical, and plastid pigment indexes of tobacco leaves Chemical composition determination The main chemical composition indices included total sugar, reducing sugar, total nitrogen, nicotine, protein, starch, potassium, chlorine, and phenolic compounds

These two brands were employed because each cigarette has the same diameters (0.8 cm), lengths without a filter (5.6 cm), nicotine levels (0.6 mg), and tar levels (8 mg)