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coli BL21(DE3) cys cells cotransformed with plasmids pCGPx3M and pMazF (TaKaRa) were grown at 37C in 400 mL of MM medium (modified M9 minimal medium) supplemented with ampicillin (100 g/mL), kanamycin (25 g/mL), chloramphenicol (25 g/mL) and Cys (50 g/mL) on a shaker

the other received the same diet supplemented with 300 mg/kg ALC for an additional 8 weeks (HCD + ALC group

2 Connect with a provider After evaluating your consultation, a provider licensed in your state will prescribe treatment (if clinically appropriate)

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