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After two PBS washes, the cells were treated with RNase A (Gibco, Grand Island, NY) for 30 min at 4 C to remove RNA and ensure only DNA is stained with propidium iodide (PI) (Sigma-Aldrich)

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Also, oxidative stress causes a disturbance in the function of pancreatic beta cells, and L-carnitine can improve the function of these cells and increase their efficiency by reducing the level of oxidative stress (67, 96)

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Bacteriostatic water includes 0.9% Benzyl Alcohol as an antimicrobial preservative